Kinetic behavior of phosphotriesterase and its non-covalent complexes with polyelectrolyte in systems with polar and non-polar organic solvents
作者： V.S. Sergeeva , E.N. Efremenko , G.M. Kazankov , A.K. Gladilin , S.D. Varfolomeev
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摘要: Soluble phosphotriesterase from E. coli DH5α together with cells the activity were co-immobilized into poly(vinyl alcohol) (PVA) cryogel and studied in water/organic systems polar non-polar organic solvents. The was competitively inhibited by inhibition constant correlated dielectric (e) of solvent. rate enzyme-catalyzed reaction biphasic solvent/water independent ratio hydrophobicity Formation non-covalent complexes polyelectrolytes suggested to enhance resistance towards inactivation solvents their homogeneous mixtures water.
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