Development and characterization of a human orthotopic neuroblastoma xenograft.
作者： Elizabeth Stewart , Anang Shelat , Cori Bradley , Xiang Chen , Sara Federico
DOI: 10.1016/J.YDBIO.2015.02.002
关键词:
摘要: Abstract Neuroblastoma is a pediatric cancer of the developing sympathoadrenal lineage. The tumors are known to develop from adrenal gland or paraspinal ganglia and have molecular cellular features sympathetic neurons such as dense core vesicles catecholamine production. Here we present detailed molecular, cellular, genetic epigenetic characterization an orthotopic xenograft derived high-risk stage 4 neuroblastoma patient. Overall, xenografted tumor retained high risk primary showed aggressive growth metastasis in mouse. Also, genome was preserved with no additional copy number variations, structural variations aneuploidy. There were 13 missense mutations identified that not patient׳s there new nonsense mutations. None acquired genes. We also demonstrate feasibility using test standard care chemotherapy targeted therapeutics. Finally, optimized approach produce cultures xenografts for high-throughput drug screening which can be used combinations therapeutic agents neuroblastoma.
core.ac.uk
elsevier.com
sci-hub.se 参考文章(23)
Elias Drakos, Carol J. Thiele, Chand Khanna, Maria Tsokos, Jerry J. Jaboin, Biologically relevant orthotopic neuroblastoma xenograft models: primary adrenal tumor growth and spontaneous distant metastasis. in Vivo. ,vol. 16, pp. 77- 85 ,(2002)
Claudia A. Benavente, Justina D. McEvoy, David Finkelstein, Lei Wei, Guolian Kang, Yong-Dong Wang, Geoffrey Neale, Susan Ragsdale, Virginia Valentine, Armita Bahrami, Jamshid Temirov, Stanley Pounds, Jinghui Zhang, Michael A. Dyer, Cross-species genomic and epigenomic landscape of retinoblastoma Oncotarget. ,vol. 4, pp. 844- 859 ,(2013) , 10.18632/ONCOTARGET.1051
A H McGregor, A H Wyllie, M J Arends, Apoptosis is inversely related to necrosis and determines net growth in tumors bearing constitutively expressed myc, ras, and HPV oncogenes. American Journal of Pathology. ,vol. 144, pp. 1045- 1057 ,(1994)
Noémie Braekeveldt, Caroline Wigerup, David Gisselsson, Sofie Mohlin, My Merselius, Siv Beckman, Tord Jonson, Anna Börjesson, Torbjörn Backman, Irene Tadeo, Ana P. Berbegall, Ingrid Öra, Samuel Navarro, Rosa Noguera, Sven Påhlman, Daniel Bexell, Neuroblastoma Patient-Derived Orthotopic Xenografts Retain Metastatic Patterns and Geno- and Phenotypes of Patient Tumours. International Journal of Cancer. ,vol. 136, pp. 252- 261 ,(2015) , 10.1002/IJC.29217
Lova Segerström, Ninib Baryawno, Baldur Sveinbjörnsson, Malin Wickström, Lotta Elfman, Per Kogner, John Inge Johnsen, Effects of small molecule inhibitors of PI3K/Akt/mTOR signaling on neuroblastoma growth in vitro and in vivo International Journal of Cancer. ,vol. 129, pp. 2958- 2965 ,(2011) , 10.1002/IJC.26268
Alexandre Chlenski, Shuqing Liu, Susan L. Cohn, The regulation of angiogenesis in neuroblastoma Cancer Letters. ,vol. 197, pp. 47- 52 ,(2003) , 10.1016/S0304-3835(03)00082-X
Markus Guba, Philipp von Breitenbuch, Markus Steinbauer, Gudrun Koehl, Stefanie Flegel, Matthias Hornung, Christiane J. Bruns, Carl Zuelke, Stefan Farkas, Matthias Anthuber, Karl-Walter Jauch, Edward K. Geissler, Rapamycin inhibits primary and metastatic tumor growth by antiangiogenesis: involvement of vascular endothelial growth factor Nature Medicine. ,vol. 8, pp. 128- 135 ,(2002) , 10.1038/NM0202-128
Mei Zeng, Jiang-Ning Zhou, Roles of autophagy and mTOR signaling in neuronal differentiation of mouse neuroblastoma cells Cellular Signalling. ,vol. 20, pp. 659- 665 ,(2008) , 10.1016/J.CELLSIG.2007.11.015
Jinghui Zhang, Li Ding, Linda Holmfeldt, Gang Wu, Sue L Heatley, Debbie Payne-Turner, John Easton, Xiang Chen, Jianmin Wang, Michael Rusch, Charles Lu, Shann-Ching Chen, Lei Wei, J Racquel Collins-Underwood, Jing Ma, Kathryn G Roberts, Stanley B Pounds, Anatoly Ulyanov, Jared Becksfort, Pankaj Gupta, Robert Huether, Richard W Kriwacki, Matthew Parker, Daniel J McGoldrick, David Zhao, Daniel Alford, Stephen Espy, Kiran Chand Bobba, Guangchun Song, Deqing Pei, Cheng Cheng, Stefan Roberts, Michael I Barbato, Dario Campana, Elaine Coustan-Smith, Sheila A Shurtleff, Susana C Raimondi, Maria Kleppe, Jan Cools, Kristin A Shimano, Michelle L Hermiston, Sergei Doulatov, Kolja Eppert, Elisa Laurenti, Faiyaz Notta, John E Dick, Giuseppe Basso, Stephen P Hunger, Mignon L Loh, Meenakshi Devidas, Brent Wood, Stuart Winter, Kimberley P Dunsmore, Robert S Fulton, Lucinda L Fulton, Xin Hong, Christopher C Harris, David J Dooling, Kerri Ochoa, Kimberly J Johnson, John C Obenauer, William E Evans, Ching-Hon Pui, Clayton W Naeve, Timothy J Ley, Elaine R Mardis, Richard K Wilson, James R Downing, Charles G Mullighan, None, The genetic basis of early T-cell precursor acute lymphoblastic leukaemia Nature. ,vol. 481, pp. 157- 163 ,(2012) , 10.1038/NATURE10725
Tal Teitz, Jennifer J. Stanke, Sara Federico, Cori L. Bradley, Rachel Brennan, Jiakun Zhang, Melissa D. Johnson, Jan Sedlacik, Madoka Inoue, Ziwei M. Zhang, Sharon Frase, Jerold E. Rehg, Claudia M. Hillenbrand, David Finkelstein, Christopher Calabrese, Michael A. Dyer, Jill M. Lahti, Preclinical Models for Neuroblastoma: Establishing a Baseline for Treatment PLoS ONE. ,vol. 6, pp. e19133- ,(2011) , 10.1371/JOURNAL.PONE.0019133