Purification of two forms of the associated 3-dehydroquinate hydro-lyase and shikimate:NADP+ oxidoreductase in Phaseolus mungo seedlings
作者： Tomokazu Koshiba
DOI: 10.1016/0005-2744(78)90317-0
关键词:
摘要: Two associated enzymes, 3-dehydroquinate hydro-lyase (EC 4.2.1.10) and shikimate:NADP+ oxidoreductase 1.1.1.25), have been purified from Phaseolus mungo seedlings. These enzymes were 6900- 9700-fold, respectively, but they not separable. Moreover, two activity bands of the detected after polyacrylamide gel electrophoresis peaks also activity. The forms showed only small differences in molecular weight, Km value, pH optimum responses to some inhibitors.
elsevier.com
sci-hub.se 参考文章(18)
D BALINSKY, DD DAVIES, Aromatic biosynthesis in higher plants. 3. Preparation and properties of dehydroquinase. Biochemical Journal. ,vol. 80, pp. 300- 304 ,(1961) , 10.1042/BJ0800300
Alain M. Boudet, Raymond L�cussan, Annie Boudet, [Characterization and properties of two dehydroquinate hydro-lyases in higher plants]. Planta. ,vol. 124, pp. 67- 75 ,(1975) , 10.1007/BF00390069
A M Boudet, R Lécussan, [Studies on the association of 5-dehydroquinate hydro-lyase and shikimate: NADP(+)-oxidoreductase in higher plants]. Planta. ,vol. 119, pp. 71- 79 ,(1974) , 10.1007/BF00390823
2. Alicyclic acid metabolism in plants Enzymes related to the shikimate pathway in developing mung bean seedlings Plant and Cell Physiology. ,vol. 9, pp. 451- 460 ,(1968) , 10.1093/OXFORDJOURNALS.PCP.A079363
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
Shizuo Hattori, Seiichi Yoshida, Masao Hasegawa, Occurrence of Shikimic Acid in the Leaves of Gymnosperms Physiologia Plantarum. ,vol. 7, pp. 283- 289 ,(1954) , 10.1111/J.1399-3054.1954.TB07577.X
Jerry L. Hedrick, Alan J. Smith, Size and charge isomer separation and estimation of molecular weights of proteins by disc gel electrophoresis Archives of Biochemistry and Biophysics. ,vol. 126, pp. 155- 164 ,(1968) , 10.1016/0003-9861(68)90569-9
A. Boudet, Mise en evidence chez les vegetaux superieurs d'une association enzymatique bifonctionnelle intervenant dans la biosynthese de l'acide shikimique FEBS Letters. ,vol. 14, pp. 257- 261 ,(1971) , 10.1016/0014-5793(71)80631-2
Hans Lineweaver, Dean Burk, The Determination of Enzyme Dissociation Constants Journal of the American Chemical Society. ,vol. 56, pp. 658- 666 ,(1934) , 10.1021/JA01318A036
Gunter M. Rothe, Intracellular compartmentation and regulation of two shikimate dehydrogenase isoenzymes in Pisum sativum Zeitschrift für Pflanzenphysiologie. ,vol. 74, pp. 152- 159 ,(1974) , 10.1016/S0044-328X(74)80168-6