Binding of wogonin to human serum albumin: a common binding site of wogonin in subdomain IIA

摘要: Abstract The binding of wogonin to human serum albumin (HSA) has been studied by spectroscopic method including circular dichroism (CD), infrared spectra (IR) and fluorescence spectra. properties HSA were examined in presence the intensity was significantly decreased wogonin. parameters from decreasing fluoremetric titrations. Stern–Volmer plots indicated that at 296, 303, 310 K is characterized one site with constant S–V 1.872 × 10 5 , 1.561 × 10 1.392 × 10 respectively, which are good agreement results Scatchard plots. process exothermic, enthalpy driven spontaneous, as thermodynamic analyses, major part energy hydrophobic interaction, consistent result molecule modelling study, there also a numbers hydrogen bonds between HSA. Furthermore, displacement experiments indicate can bind subdomain IIA, is, I HSA, study.