The genetic diversity of human papillomavirus types from the species Gammapapillomavirus 15: HPV135, HPV146, and HPV179.

摘要: Objectives To determine the prevalence, viral load, tissue tropism, and genetic variability of novel human papillomavirus (HPV) type 179, which is etiologically associated with sporadic cases common warts in immunocompromised patients, phylogenetically related HPV types 135 146. Methods The representative collection 850 HPV-associated clinical samples (oral/nasopharyngeal/anal, archival specimens oral/oropharyngeal/conjunctival/cervical/skin cancer, benign lesions larynx/conjunctiva/skin, eyebrows), obtained from immunocompetent individuals, was tested for presence HPV179, HPV135, HPV146 using type-specific real-time PCRs. assess diversity HPVs investigated non-coding long control region (LCR), several highly sensitive nested PCR protocols were developed each type. HPV179 additionally determined 12 isolates different anatomical sites an only patient included study. Results detected 1.4, 2.0, 1.5% tested, respectively, no preference cutaneous or mucosal epithelial cells. One (with five single nucleotide polymorphisms; SNPs), four one to six eight SNPs) variants HPV146, identified among eligible samples. exhibited identical LCR sequence, suggesting that can cause generalized infections. Conclusions have a mucocutaneous tropism are infections individuals. Because majority mutations found outside major functional domains respective LCRs, we assume pathogenically do not differ their prototypes. In addition, association between specific infection and/or neoplasms.