Identification of Klebsiella pneumoniae Carbapenemase-producing Klebsiella oxytoca in Clinical Isolates in Tehran Hospitals, Iran by Chromogenic Medium and Molecular Methods.

摘要: Abstract Objectives Production of carbapenemase, especially Klebsiella pneumoniae carbapenemases (KPC), is one the antibiotic resistance mechanisms Enterobacteriaceae such as oxytoca . This study aimed to investigate and identify KPC-producing K. isolates using molecular phenotypic methods. Methods A total 75 were isolated from various clinical samples, verified after performing standard microbiological tests a polymerase chain reaction (PCR) method. An susceptibility test was performed disc diffusion method according Clinical Laboratory Standards Institute guidelines. CHROMagar KPC chromogenic culture media used examine confirm production carbapenemase enzyme in isolates; addition, PCR evaluate presence bla gene strains. Results Of 75  isolates, multidrug resistant strain urine hospitalized woman. examined assess its ability produce enzyme; it produced colony with blue metallic color on media. In confirmed by PCR. After sequencing, deposited GenBank. Conclusion To date, many cases Enterobacteriaceae, particular , have been reported different countries; there are also some reports identification Therefore, prevent outbreak nosocomial infections, early detection, control, prevention spread these strains great importance.