Co-culture Systems of Drug-Treated Acute Myeloid Leukemia Cells and T Cells for In Vitro and In Vivo Study

摘要: Summary A combination of immunotherapy and chemotherapy strategies could strengthen antitumor effects. This protocol elucidates a robust method via co-culturing drug pre-treated acute myeloid leukemia cells with CD3+ T cells, derived from leukoreduction system chambers, for in vitro vivo study. We optimized several aspects the procedures, including timing treatment, quantification tumor cells, approach T cells treatment vivo. enables readouts interplay between drugs T cells. For complete details on use execution this protocol, please refer to Su et al. (2020) .