Effect of carbamate esters on neurite outgrowth in differentiating human SK-N-SH neuroblastoma cells.
作者： Ping-An Chang , Yi-Jun Wu , Wei Li , Xin-Fu Leng
DOI: 10.1016/J.CBI.2005.09.005
关键词:
摘要: Abstract Carbamate esters are widely used as pesticides and can cause neurotoxicity in humans animals; the exact mechanism is still unclear. In present investigation, effects of carbamates at sublethal concentration on neurite outgrowth cytoskeleton well activities acetylcholinesterase (AChE) neuropathy target esterase (NTE) differentiating human SK-N-SH neuroblastoma cells were studied. The results showed that 50 μM either aldicarb or carbaryl significantly decreased length retinoic acid-induced differentiation cells, compared to treated with vehicle. Western blot analyses revealed neither carbamate had significant levels actin, total neurofilament high molecular proteins (NF-H). However, increased NF-H phosphorylation was observed following treatment. These changes may represent a useful vitro marker within simple model neuronal cell differentiation. Furthermore, activity AChE, but not NTE, inhibited by which suggested cytoskeletal protein induced associated inhibition AChE NTE.
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