作者: Kiyoshi Yamauchi , Shigekazu Kurakake , Takahiro Morosawa , Mami Kondo , Reina Uno
DOI: 10.3106/041.039.0402
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摘要: Abstract. In order to establish methodology for population estimation of Asiatic black bears using the hair-trapping method, devices were located within a study area, and basic data collected determine optimal survey period hair sampling. Bear hairs classified as three types (guard hair; G, underfur; U, intermediate I) depending on hair-bulb thickness. After DNA extraction, six microsatellite loci amelogenin locus amplified in identify individual sex, respectively. The number by decreased dramatically late August, while those that had lost part bulb increased after September. success rate genetic analysis was over 90% prior month July rapidly August. Based results generalized linear model, it determined sampling session most significant impact all explanatory...