Turgor pressure responses of a gram-negative bacterium to antibiotic treatment, measured by collapse of gas vesicles.

摘要: The internal hydrostatic pressure of Ancylobacter aquaticus was measured by collapsing the gas vesicles with an externally applied pressure. Turgor in conjunction various antibiotic treatments to elucidate some aspects biophysics gram-negative cell wall function. Differences effects these drugs either alone or combination other were related known biochemical activities drugs. Our previous work, demonstrating a heterogeneous cellular response beta-lactam antibodies, confirmed and extended. Most growth-inhibiting antibiotics resulted cells (those component I) developing higher pressure, while remainder II) lost turgor. Although fraction each varied little from subculture subculture, it did not vary time choice treatment. Mecillinam gave nearly monophasic response. All blocking macromolecular synthesis curves. 50% collapse cases, however, lower higher, same as control.