Immobilised pectinase efficiency in the depolymerisation of pectin in a model solution and apple juice

作者: C. Dinnella , A. Stagni , G. Lanzarini , M. Laus

DOI: 10.1016/S0921-0423(96)80341-8

关键词:

摘要: Abstract A commercial pectinase, immobilised on appropriately functionalised γ-alumina spheres, was loaded in a packed bed reactor and employed to depolymerise the pectin contained model solution apple juice. The activity of immobilized enzyme tested several batch reactions compared with one free enzyme. successful juice depectinisation obtained using pectinase system. In addition, an endopolygalacturonase from Kluyveromyces marxianus , previously purified single-step process coreshell microspheres specifically prepared, same active support efficiency resulting catalyst tested.

参考文章(8)
Martin F Chaplin, John Frederick Kennedy, Carbohydrate analysis: a practical approach Published in <b>1986</b> in Oxford by IRL press. ,(1986)
M.-A. Coletti-Previero, A. Previero, Alumina-phosphate complexes for immobilization of biomolecules. Analytical Biochemistry. ,vol. 180, pp. 1- 10 ,(1989) , 10.1016/0003-2697(89)90080-8
Frances M. Barnby, F.F. Morpeth, D.L. Pyle, Endopolygalacturonase production from Kluyveromyces marxianus. I. Resolution, purification, and partial characterisation of the enzyme Enzyme and Microbial Technology. ,vol. 12, pp. 891- 897 ,(1990) , 10.1016/0141-0229(90)90028-O
Caterina Dinnella, Gaetano Lanzarini, Marcella Zannoni, Michele Laus, Immobilization and reactivity of enzymes on functional particles prepared by dispersion polymerization Macromolecular Rapid Communications. ,vol. 15, pp. 909- 915 ,(1994) , 10.1002/MARC.1994.030151201
Milena Kmínková, Jirí Kučera, Comparison of pectolytic enzymes covalently bound to synthetic ion exchangers using different methods of binding Enzyme and Microbial Technology. ,vol. 5, pp. 204- 208 ,(1983) , 10.1016/0141-0229(83)90096-0
Pedro Lozano, Arturo Manjón, JoséL. Iborra, Manuel Cánovas, Félix Romojaro, Kinetic and operational study of a cross-flow reactor with immobilized pectolytic enzymes Enzyme and Microbial Technology. ,vol. 12, pp. 499- 505 ,(1990) , 10.1016/0141-0229(90)90065-X
Peter Albersheim, Ursula Killias, Studies relating to the purification and properties of pectin transeliminase. Archives of Biochemistry and Biophysics. ,vol. 97, pp. 107- 115 ,(1962) , 10.1016/0003-9861(62)90050-4