An animal cell culture monitoring system using a smartphone-mountable paper-based analytical device

摘要: Abstract We developed a simple and low-cost cell culture monitoring system utilizing paper-based analytical device (PAD) smartphone. The PAD simultaneously analyses glucose lactate concentrations in the medium. Focusing on fact that animal cells consume produce under anaerobic conditions, oxidase- horseradish peroxidase (HRP) enzyme-mediated colorimetric assays were integrated into PAD. was designed to have three laminated layers. By using double-sided adhesive tape as middle layer wax coating, bifurcated fluidic channel prepared manipulate sample flow. At inlet outlets of channel, drop zone two detection zones for lactate, respectively, positioned. When solution is loaded onto zone, it flows through hydrophilic via capillary force. Upon reaching reacts with oxidases (GOx LOx) HRP, immobilized along colorless chromophores. Trinder’s reaction, chromophore converted blue-colored product, generating concentration-dependent signal. With gadget aid image acquisition, positioned smartphone-embedded camera. Images acquired mobile application color intensities quantified sensor signals. For assay GOx/HRP format, we obtained limit (LOD ∼0.3 mM) quantification (LOQ ∼0.9 mM) values dynamic range from 0.3 8.0 mM glucose. LOx/HRP, LOD (0.02 mM) LOQ (0.06 mM) registered 0.02 0.50 mM lactate. device, simultaneous media conducted, exhibiting highly accurate reproducible results. Based results, propose optical sensing feasible practical culture.