Nitric oxide-releasing poly(lactic-co-glycolic acid)-polyethylenimine nanoparticles for prolonged nitric oxide release, antibacterial efficacy, and in vivo wound healing activity.
作者： Jin-Wook Yoo , Hasan Nurhasni , Jiafu Cao , Moonjeong Choi , Il Kim
DOI: 10.2147/IJN.S82199
关键词:
摘要: Nitric oxide (NO)-releasing nanoparticles (NPs) have emerged as a wound healing enhancer and novel antibacterial agent that can circumvent antibiotic resistance. However, the NO release from NPs over extended periods of time is still inadequate for clinical application. In this study, we developed NO-releasing poly(lactic-co-glycolic acid)-polyethylenimine (PEI) (NO/PPNPs) composed acid) PEI/diazeniumdiolate (PEI/NONOate) prolonged release, efficacy, activity. Successful preparation PEI/NONOate was confirmed by proton nuclear magnetic resonance, Fourier transform infrared spectroscopy, ultraviolet/visible spectrophotometry. NO/PPNPs were characterized particle size, surface charge, loading. The showed profile 6 days without any burst release. exhibited potent bactericidal efficacy against methicillin-resistant Staphylococcus aureus (MRSA) Pseudomonas aeruginosa concentration-dependently ability to bind on bacteria. We also found released mediates not toxic healthy fibroblast cells. Furthermore, accelerated epithelialization in mouse model MRSA-infected wound. Therefore, our results suggest presented study could be suitable approach treating wounds various skin infections.
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