Production of Monoclonal Antibodies in Glycoengineered Pichia pastoris

摘要: Although improvements in antibody expression by mammalian cells are nearing maturation, efforts to improve efficacy through glycoengineering rapidly expanding. For example, the production of full length monoclonal antibodies with uniform human N-linked glycans glycoengineered yeast has been used optimize effector function. The platform not only enables elucidation structure function relationships but also offers a robust and economically viable alternative cell expression. This chapter provides an overview glycobiology, engineering P. pastoris secrete recombinant proteins as well bioprocess considerations based system.