Glycoproteoform Profiles of Individual Patients' Plasma Alpha-1-Antichymotrypsin are Unique and Extensively Remodeled Following a Septic Episode.

摘要: Sepsis and septic shock remain the leading causes of death in intensive care units (ICUs), yet pathogenesis originating from inflammatory response during sepsis remains ambiguous. Acute-phase proteins are typically highly glycosylated, nature glycans have been linked to incidence severity such responses. To further build upon these findings we here monitored, longitudinal changes plasma proteome and, molecular detail, glycoproteoform profiles alpha-1-antichymotrypsin (AACT) extracted ten individual patients. For each patient included four different time-points, including post-operative (before sepsis) following discharge ICU. We isolated AACT depleted for albumin, IgG serotransferrin used high-resolution native mass spectrometry qualitatively quantitatively monitor multifaceted glycan microheterogeneity desialylated AACT, which allowed us how reflected patient's physiological state. Although observed a general trend remodeling profiles, e.g. increased fucosylation branching/LacNAc elongation, exhibited unique features responses, providing resilient proof-of-concept importance personalized profiling. Importantly, that induced by did not readily subside after