Functional characterization of gibberellin oxidases from cucumber, Cucumis sativus L.
作者： Maria João Pimenta Lange , Anja Liebrandt , Linda Arnold , Sara-Miriam Chmielewska , André Felsberger
DOI: 10.1016/J.PHYTOCHEM.2013.02.006
关键词:
摘要: Abstract Cucurbits have been used widely to elucidate gibberellin (GA) biosynthesis. With the recent availability of genome sequence for economically important cucurbit Cucumis sativus, data became available all genes potentially involved in GA biosynthesis this species. Sixteen cDNAs were cloned from root and shoot 3-d 7-d old seedlings mature seeds C. sativus. Two code 7-oxidases (CsGA7ox1, -2), five 20-oxidases (CsGA20ox1, -2, -3, -4, -5), four 3-oxidases (CsGA3ox1, -4), another 2-oxidases (CsGA2ox1, -5). Their enzymatic activities investigated by heterologous expression Escherichia coli incubation cell lysates with 14C-labelled, D2-labelled, or unlabelled GA-substrates. The two converted GA12-aldehyde GA12 efficiently. CsGA7ox1 GA14, 15α-hydroxyGA12, further 15α-hydroxyGA14. CsGA7ox2 its 12α-hydroxylated analogue GA111. All GA9 as a major product, GA25 minor product. oxidized C19-GA GA4 only In addition, three them (CsGA3ox2, -4) C20-GA GA14. CsGA2ox1, -4 C19-GAs GA34 GA51, respectively. CsGA2ox2, GA51 respective GA-catabolites. addition C19-GAs, CsGA2ox4 also GA110. contrast, CsGA2ox5 C20 GA110 sole As shown CsGA20ox1 CsGA3ox1, similar reactions catalysed 13-hydroxlyated GAs substrates. It is likely that these enzymes are responsible 13-hydroxylated vivo occur at low levels cucumber.
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