Blood-Based miRNA Preparation for Noninvasive Biomarker Development
作者： Svenja Debey-Pascher , Jing Chen , Thorsten Voss , Andrea Staratschek-Jox
DOI: 10.1007/978-1-61779-427-8_22
关键词:
摘要: This chapter describes several methods for the isolation of miRNAs from peripheral whole blood samples or constituent fractions thereof, such as mononuclear cells, plasma, and serum. The described here are recently introduced protocols dedicated to total RNAs including small RNAs, e.g., miRNeasy Kit PAXgene Blood miRNA Kit, alternatively enrichment low-molecular-weight RNA (LMW RNA) using Kit. Furthermore, modifications classical purification facilitate recovery highlighted.
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