Chapter 7 Molecular Associations and Membrane Domains

作者: Michael Edidin

DOI: 10.1016/S0070-2161(08)60169-0

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摘要: Publisher Summary This chapter focuses on the models of membrane organization. The most widely cited model organization cell membranes is termed “fluid mosaic.” It emphasizes mobility and autonomy lipids proteins implies a role for molecular in function. Indeed, this aspect has been basis proposals that reactions among may be coupled by collision diffusing species signaling from surface require clustering mobile receptors antigens or hormones. discusses results with technique, fluorescence photobleaching recovery (FPR) was developed to quantitate D lat how technique measuring scale micrometers per minute can used demonstrate associations immobilization­­­­––that is, way which FPR investigate spatial temporal surfaces. measures lateral proteins, readily show “grainy,” differentiated into regions specialized composition Examples as qualitative tool detecting A minimum two cross-linking antibodies are required, ideally third, irrelevant monoclonal antibody should available effects specific pair molecules interest.

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