Muscle cells produce a low molecular weight factor with anti-cancer activity

摘要: The present study describes a new low molecular weight factor released by muscle cells, which inhibits proliferation of tumor cells in vitro and vivo, is highly specific towards has no observable effect on normal cells' proliferation. What first prompted us to investigate this was the observation that metastases are extremely rare striated muscles. Co-culturing with malignant led marked morphological alterations latter, contrast same when incubated without cells. A conditioned medium prepared its tested variety This (CM) inhibited cell lines murine (B16 melanoma, Madison 109 lung carcinoma, MCA-105 sarcoma, ESB lymphoma), or human origin (HTB-38 adenocarcinoma, T47D breast CX1 colon carcinoma). (bone marrow fetal liver erythroid cells) not affected CM. Flow cytometric analysis B16 melanoma following incubation CM revealed 63% +/- 12 were G0/G1 phase cycle, compared 47.8% 8 (not conditioned) only. activity certain fractions thereof also demonstrated vivo: they prevented growth mice inoculated intraperitoneally sarcoma Partial purification active component non-proteinaceous compound about 500 D. results clearly suggest produce can selectively inhibit vivo. neither species nor specific.