Genomic DNA methylation decreases in response to moderate folate depletion in elderly women

摘要: Background Methylation of genomic DNA is dependent on an adequate supply folate coenzymes. Previous data support the hypothesis that abnormal methylation plays integral role in carcinogenesis. To date, no studies assessing effect inadequate status older women (aged >63 y) have been reported. Objective The moderate depletion followed by repletion leukocyte was investigated elderly 60-85 to evaluate whether could be used as a functional indicator status. Design Healthy, postmenopausal (n = 33) consumed moderately folate-depleted diet (118 microg folate/d) for 7 wk, wk with 200 or 415 microg/d, each provided 2 different dietary treatments total 4 treatment groups 30). Leukocyte determined basis ability incorporate [(3)H]methyl from labeled S:-adenosylmethionine vitro assay. Results Incorporation increased significantly (P: 0.0025) response depletion, suggesting undermethylation DNA. No significant changes were detected incorporation any group over 7-wk period compared postdepletion values. Conclusions may depleted slow diets observed suggests normalization after delayed women.