Human fibroblast matrices bio-assembled under macromolecular crowding support stable propagation of human embryonic stem cells.

作者: Yanxian Peng , Michael Thomas Bocker , Jennifer Holm , Wei Seong Toh , Christopher Stephen Hughes

DOI: 10.1002/TERM.1560

关键词:

摘要: … This study showed that human ECM bio-assembled in vitro using macromolecular crowding and decellularized with the anionic detergent deoxycholate could support hESC culture for …

参考文章(40)
P W Andrews, N C Dracopoli, J Føgh, D Simon, C Carlin, I Damjanov, G S Banting, Pluripotent embryonal carcinoma clones derived from the human teratocarcinoma cell line Tera-2. Differentiation in vivo and in vitro. Laboratory Investigation. ,vol. 50, pp. 147- 162 ,(1984)
CZC Chen, YX Peng, ZB Wang, PV Fish, JL Kaar, RR Koepsel, AJ Russell, RR Lareu, M Raghunath, The Scar‐in‐a‐Jar: studying potential antifibrotic compounds from the epigenetic to extracellular level in a single well British Journal of Pharmacology. ,vol. 158, pp. 1196- 1209 ,(2009) , 10.1111/J.1476-5381.2009.00387.X
J. P. Annes, Making sense of latent TGFβ activation Journal of Cell Science. ,vol. 116, pp. 217- 224 ,(2003) , 10.1242/JCS.00229
Margaret S. Inokuma, Jerrod Denham, Kathaleen Golds, Pratima Kundu, Joseph D. Gold, Melissa K. Carpenter, Chunhui Xu, Feeder-free growth of undifferentiated human embryonic stem cells. Nature Biotechnology. ,vol. 19, pp. 971- 974 ,(2001) , 10.1038/NBT1001-971
Chris S Hughes, Lynne M Postovit, Gilles A Lajoie, None, Matrigel: A complex protein mixture required for optimal growth of cell culture Proteomics. ,vol. 10, pp. 1886- 1890 ,(2010) , 10.1002/PMIC.200900758
Samira M. Azarin, Sean P. Palecek, Matrix Revolutions: A Trinity of Defined Substrates for Long-Term Expansion of Human ESCs Cell Stem Cell. ,vol. 7, pp. 7- 8 ,(2010) , 10.1016/J.STEM.2010.06.008
Paromita Deb-Rinker, Dao Ly, Anna Jezierski, Marianna Sikorska, P. Roy Walker, Sequential DNA Methylation of the Nanog and Oct-4 Upstream Regions in Human NT2 Cells during Neuronal Differentiation Journal of Biological Chemistry. ,vol. 280, pp. 6257- 6260 ,(2005) , 10.1074/JBC.C400479200
Simone Bork, Stefan Pfister, Hendrik Witt, Patrick Horn, Bernhard Korn, Anthony D. Ho, Wolfgang Wagner, DNA methylation pattern changes upon long-term culture and aging of human mesenchymal stromal cells. Aging Cell. ,vol. 9, pp. 54- 63 ,(2010) , 10.1111/J.1474-9726.2009.00535.X
Clarice Chen, Felicia Loe, Anna Blocki, Yanxian Peng, Michael Raghunath, Applying macromolecular crowding to enhance extracellular matrix deposition and its remodeling in vitro for tissue engineering and cell-based therapies ☆ Advanced Drug Delivery Reviews. ,vol. 63, pp. 277- 290 ,(2011) , 10.1016/J.ADDR.2011.03.003