Development and characterization of a live varicella vaccine (Oka strain).

摘要: A live varicella vaccine (Oka strain) was developed by serial passage of the Oka strain isolated in our laboratory, human embryonic lung cells (11 times at 34 C) and guinea pig embryo (12 37 C). It is slightly temperature sensitive 39 C shows a higher ratio infectivity to than wild-type strains. The DNA digest with Hpa I enzyme contained one unique fragment (K), although its mobility differed only from that corresponding Studies clinical zoster virus (VZV) isolates vaccinees indicated tests on fibroblasts (GPEF) (HuEF) profile are useful for differentiation showed stable immunogenicity during least 15 further repeated passages diploid cells, character which seems helpful production large quantity practical use.