Eradication of Eperythrozoon coccoides with oxophenarsine in normal and drug-resistant lines of Plasmodium berghei in mice.

摘要: A parent line of Plasmodium berghei and three drug-resistant derivatives inadvertently became mixed with Eperythrozoon coccoides. Oxophenarsine hydrochloride (Mapharsen?) was used to eradicate E. The cleared by repeated passage through mice maintained on 0.05% or 0.1% drug diets given 10 mg/kg doses the daily parenteral administration. Examinations several months after drug-diet treatment indicated that plasmodia were not resistant oxophenarsine. also in clearing a highly cycloguanil hydrochloride. P. lines 4,4'-diaminodiphenylsulfone chloroquine administration 5 daily. maintenance disease agents serially transferring blood tissues provides opportunities for be such natural organisms as type then induced may differ markedly from produced experimental agent alone. Stansly (1965) has reviewed instances synergism mouse hepatitis, lymphocytic choriomeningitis, lactic dehydrogenase. He independent induction splenomegaly coccoides, which greatly complicated studies neoplastic diseases mice. competitive action coccoides been described Kretschmar (1963) Peters Babesia rodhaini (1965). detected recently our lines. Both theoretical considerations practical difficulties emphasized importance freeing these parasites contaminant. Neoarsphenamine cure (Bruynoghe Vassiliadis, 1929; Thurston, 1953). elimination sequential neoarsphenamine oxytetracycline. Inasmuch is readily available this country, we elected try related trivalent arsenical oxophenarsine This report deReceived publication 14 March 1966. 674 scribes successful use berghei. MATERIALS AND METHODS 12A strain work female CF-1 All sprayed disinfectant (1:300 Kreso Dip?) upon arrival no lice ever seen any animals. Infections passed weekly inoculating parasitized intraperitoneally. In some known inoculum (15 X 106 cells) injected but usually 0.1 0.3 ml whole used, roughly accordance parasite density. included had induced, long periods partially effective treatment, grow large drugs subcutaneously (Thompson et al., 1965a, b). follows: 4,6diamino1( p-chlorophenyl ) -1,2-dihydro-2,2-dimethyl-s-triazine (cycloguanil HC1), 25 mg/kg; (DDS), diphosphate, 30 mg/kg. Each more than 30-fold homologous line. serial treated (usually five ten), schedule designed provide exposure drugs. infection side group ten untreated (3-amino-4-hydroxyphenyl arsineoxide hydrochloride, Mapharsen?) either powdered diet ad lib. parenterally. first dose subcutaneously, avoid local effects during same day into periThis content downloaded 157.55.39.220 Fri, 02 Sep 2016 05:18:44 UTC subject http://about.jstor.org/terms THOMPSON BAYLES-ERADICATION OF EPERYTHROZOON FROM BERGHEI MICE 675 toneal cavity; all subsequent intraperitoneally, skin lesions at injection site high doses. dosages concentrations expressed terms free base. Parenteral aqueous preparations volumes ml/kg body weight. assessed examining Giemsa-stained films. Smears made 5th mostly 7th-day smears routinely studies.