RNA-dependent replication, transcription, and persistence of brome mosaic virus RNA replicons in S. cerevisiae
作者： M JANDA
DOI: 10.1016/0092-8674(93)90584-D
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摘要: Abstract This paper shows that yeast can be a host for the replication of higher eukaryotic viral genome. Brome mosaic virus (BMV) is member alphavirus-like superfamily animal and plant positive strand RNA viruses. Yeast expressing BMV genes 1a 2a support RNA-dependent transcription RNA3 derivatives. derivatives with coat gene replaced by reporter expressed in fashion dependent on expression trans signals cis. carrying URA3 complemented ura3 − to prototrophy were maintained as persistent episomes. Thus, all cellular factors essential must present yeast, genetics should facilitate their identification.
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