Excessive Production of IFN-γ in Patients with Systemic Lupus Erythematosus and Its Contribution to Induction of B Lymphocyte Stimulator/B Cell-Activating Factor/TNF Ligand Superfamily-13B

摘要: Expression and immunological significance of IFN-gamma, a pivotal cytokine in murine lupus, have not been clearly demonstrated human systemic lupus erythematosus (SLE). In the present study we investigated expression IFN-gamma peripheral blood T cells from patients with SLE its role production soluble B lymphocyte stimulator (sBLyS). Peripheral expressed significantly larger amounts response to stimulation anti-CD3 mAb plus anti-CD28 than those normal controls as shown by three analytical methods, including ELISA, flow cytometry, quantitative RT-PCR. The ratio IFN-gamma-producing effector memory CD3(+)CD4(+) CD3(+)CD8(+) populations was higher that controls. T-box (T-bet) mRNA/GATA-binding protein-3 (GATA-3) mRNA cell culture supernatants contained sBLyS-inducing activity controls; this almost completely inhibited addition anti-human mAb. Percentages BLyS-expressing monocytes were Monocytes produced sBLyS Taken together, these data strongly indicate overexpression contributes immunopathogenesis via induction monocytes/macrophages, which would promote activation maturation.