MicroRNA and mRNA expression profiling in rat acute respiratory distress syndrome

摘要: Acute respiratory distress syndrome (ARDS) is characterized by pulmonary epithelial injury and extensive inflammation of the parenchyma. Systematic analyses microRNA (miRNA) mRNA expression profiling in ARDS provide insights into understanding molecular mechanisms pathogenesis ARDS. The objective this study was to identify miRNA interactions a rat model combining microarray analyses. Rat induced saline lavage mechanical ventilation. profiles both mRNAs miRNAs were performed Microarray data further verified quantitative RT-PCR. Functional annotation on dys-regulated carried out bioinformatics analysis. 27 37 found be significantly changed. selected genes real-time PCR. down-regulated included miR-24, miR-26a, miR-126, Let-7a, b, c, f. up-regulated composed miR-344, miR-346, miR-99a, miR-127, miR-128b, miR-135b, miR-30a/b. Gene ontology functional indicated that mRNAs, such as Apc, Timp1, Sod2, involved regulation apoptosis. Bioinformatics analysis showed inverse correlation altered with their predicted target mRNAs. While Sod2 inversely correlated f., Ebf1 Apc miR-24 respectively. miR-30a/b, miR-18a targeted multiple Gabrb1, Eif2ak1, Fbln5, Tspan8 miRNAs. expressions identified miRNA-mRNA pairs may play critical roles