Amino acid sequence of mouse tenascin and differential expression of two tenascin isoforms during embryogenesis.

摘要: We have isolated cDNA clones for mouse tenascin and analyzed expression of mRNAs during embryonic development the kidney gut. The deduced amino acid sequence cDNAs shows a modular structure repeats similar to chicken human tenascin. In there are 14.5 cysteine-rich with similarity EGF repeat, followed by several type III repeat fibronectin. A longer variant contains 13 fibronectin repeats, whereas shorter splice lacks 5 that occur directly after fifth in variant. Contrary sequences, does not contain an RGD third implicated cell attachment, or any other positions. Northern hybridizations RNA from primary fibroblasts, clone M 20/1 detects two sizes close 6 8 kb. This, data presented here suggest major polypeptides arise through alternative splicing. differentially expressed development. 8-kb mRNA is more prominent than 6-kb throughout prenatal development, but postnatal ratio changes. different pattern seen developing gut where predominates embryogenesis appearing later. correspond previous protein data, which showed Mr 210,000 polypeptide earlier developmental stages larger 260,000 appears later (Aufderheide, E., P. Ekblom. 1988. J. Cell Biol. 107:2341-2349).