Intestinal permeability modulation through a polyphenol-rich dietary pattern in older subjects: MaPLE project outcomes and perspectives

摘要: In recent years, research has been focusing on strategies to counteract inflammatory processes and age-related diseases(1). During ageing, a low-grade systemic inflammation is often associated an altered intestinal permeability (IP) condition that shown promote possibly through the translocation of dietary bacterial factors into blood stream activates immune system(2).In this regard, pattern environmental could play fundamental role because their potential ability modulate inflammation, IP gut microbial ecosystem (GME). Moreover, it hypothesized bioactive compounds such as polyphenols may affect GME(3).The MaPLE project (Microbiome mAnipulation Polyphenols for managing Leakiness in Elderly) aimed investigate hypothesis polyphenol-rich diet can improve target population with beneficial changes at intestine level. To aim, randomised, controlled, cross-over intervention study (8-week versus 8-week control diet, separated by wash-out period) was carried out group older subjects (> 60 years) living well-controlled setting (i.e. nursing home). Markers related IP, oxidative stress, vascular function were investigated serum, urine and/or fecal samples. bacteria DNAemia, serum/urine metabolomics assessed. consistent nutritional evaluation standard menu (provided home) weighed food diaries performed, providing also data actual polyphenol intake during intervention. The results show there higher levels subjects, polyphenol-enriched changed serum zonulin, marker IP. addition, association between zonulin load demonstrated. Ongoing vitro vivo experiments are exploring effects different mechanisms involved. will generate new understanding modulation microbiome its interactions host.