A Sequencing Method for RNA Oligonucleotides Based on Mass Spectrometry

摘要: Synthetic oligoribonucleotides up to 22 bases have been sequenced by observing different kinetics in exonuclease-induced phosphodiester bond hydrolysis and detecting the fragments matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS). Common spectrometric sequencing methods disadvantages concerning read length, cost, specialist instrumentation using RNA as target molecule because uridine cytidine similar masses. Now we are position distinguish U C peak intensities. The method proposed has verified specific endonucleases 13C-labeled nucleotides. This new nongel-based nonlabeling strategy offers first data advantages fast accurate MALDI-TOF-MS. Preparation steps measurements performed less than 1 h.