Microinjection of rat growth-hormone gene into zebrafish egg and production of transgenic zebrafish

摘要: A plasmid containing rat growth-hormone gene was microinjected into fertilized zebrafish eggs prior to first cleavage; survival of the embryos one day after injection averaged 46% Genomic DNA extracted from presumotive transgenic fish analysed by slot-blot and Southern-blot hybridization using labelled as probe. Patterns indicated genomic, extrachromosomal, well mosaic integration. The concentration persisting extrachromosomal progressively decreased in F 1 2 generations. growth rate higher 0 generations, but lower , which may be due translent expression carrying