METHOD AND KIT FOR SYNTHESIZING NUCLEIC ACID UNDER CONSTANT TEMPERATURE CONDITIONS
作者： Wang Zhuo , Mao Rui , Du Yuguang
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摘要: The invention discloses a method and kit for synthesizing nucleic acid in constant temperature conditions. comprises the following steps: 1) providing acid, wherein 5' terminal of is provided with an Nc region capable performing N annealing on same chain, moreover, 3' competitive relation exists between at chain; 2) taking step as template, which has realizes synthetic beginning point, complementary 3) carrying out chain synthesis through polymerase catalytic displacement type reaction so to displace synthesized 2). main advantages that rapid amplification genes can be realized system single enzyme short segment (an ideal just 60 bp, shorter than LAMP minimum 120 bp).
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