Multielectrode Array (MEA) Assay for Profiling Electrophysiological Drug Effects in Human Stem Cell-Derived Cardiomyocytes
作者： Mike Clements
DOI: 10.1002/CPTX.2
关键词:
摘要: More relevant and reliable preclinical cardiotoxicity tests are required to improve drug safety reduce the cost of development. Human stem cell-derived cardiomyocytes (hSC-CMs) provide a potential model for development superior assays screening. One such hSC-CM assay that has shown significant enabling more predictive cardiac risk assessment is MEA assay. The Multi-electrode Array (MEA) an electrophysiology-based technique uses microelectrodes embedded in culture surface each well measure fluctuations extracellular field (FP) generated from spontaneously beating hSC-CMs. Perturbations recorded FP waveform can be used as unbiased method predicting identity ion channel(s) impacted on exposure. Here, higher throughput using hSC-CMs 48-well plates described profiling compound-induced effects cardiomyocyte electrophysiology. Techniques preparing monolayers methods contextualize experimental results also covered. © 2016 by John Wiley & Sons, Inc.
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