Interallelic complementation at the ubiquitous urease coding locus of soybean.

摘要: Soybean ( Glycine max [L.] Merrill) mutant aj6 carries a single recessive lesion, , that eliminates ubiquitous urease activity in leaves and callus while retaining normal embryo-specific activity. Consistently, / plants accumulated urea leaves. In crosses of by mutants at the Eu1, Eu2 Eu3 loci, F 1 individuals exhibited wild-type leaf activity, 2 segregated urease-negative individuals, demonstrating is not an allele these loci. crossed with null lacking Eu1 -encoded showed was also inactive seeds . The cross to eu4 previously assigned structural gene (R.S. Torisky, J.D. Griffin, R.L. Yenofsky, J.C. Polacco [1994] Mol Gen Genet 242: 404–414), yielded having 22% ± 11% Coding sequences for were cloned reverse transcriptase-polymerase chain reaction from wild-type, RNA. had 837-amino acid open reading frame (ORF), 87% identical urease. ORF R201C change cosegregated lack against urease-positive line, whereas G468E change. Heteroallelic interaction progeny × resulted partially restored These results confirm are affected gene. They indicate radical amino changes distinct domains can be compensated heterotrimer.