Heparin-LL37 complexes are less cytotoxic for human dental pulp cells and have undiminished antimicrobial and LPS-neutralizing abilities.

摘要: AIM To investigate whether glycosaminoglycans (GAGs) binding to high-dose LL37 eliminates its cytotoxicity dental pulp cells (hDPCs) whilst retaining undiminished antimicrobial and LPS-neutralizing abilities. METHODOLOGY hDPCs were stimulated with varying concentrations of LL37, their cell viability was analysed by MTT. Then, (10 μmol L-1 ) bound three GAGs, heparin, chondroitin sulphate hyaluronic acid, cytotoxic effects on evaluated compared. Furthermore, the ability heparin (5 μg mL-1 )-LL37 complexes, which found be less for ability, investigated. Statistical analysis performed using one-way variance (anova), followed Dunnett's test. P values below 0.05 considered significant. RESULTS significantly reduced in a dose-dependent manner (P < 0.01). within limited concentration range (2~6 μg mL-1 eliminated (P < 0.01) exerting potent against Streptococcus mutans, sobrinus, salivarius, Aggegatibacter actinomycetemcomitans Escherichia coli. exhibited similar functions (P < 0.01); however, effective highly restricted (3 μg mL-1 ). acid unable abrogate even at higher (10 100 μg mL-1 Moreover, exogenous addition LPS dose-dependently amount precipitated heparin-LL37 agarose beads (P < 0.01), released simultaneously neutralized pro-inflammatory macrophages CONCLUSIONS Heparin-LL37 complexes generated suitable ratios are easy make, broad-range materials that can neutralize providing accordance free LPS. They may potential treatment save tissue from acute inflammation exacerbated invading bacteria they release.