Plasma Membrane Proteomics of Human Breast Cancer Cell Lines Identifies Potential Targets for Breast Cancer Diagnosis and Treatment

摘要: The use of broad spectrum chemotherapeutic agents to treat breast cancer results in substantial and debilitating side effects, necessitating the development targeted therapies limit tumor proliferation prevent metastasis. In recent years, list approved has expanded, it includes both monoclonal antibodies small molecule inhibitors that interfere with key proteins involved uncontrolled growth migration cells. targeting plasma membrane been most successful date, this is reflected large representation these as targets newer therapies. view facts, experiments were designed investigate proteome a variety human cell lines representing hormone-responsive, ErbB2 over-expressing triple negative types, well benign control. Plasma membranes isolated by using an aqueous two-phase system, resulting subjected mass spectrometry analysis. Overall, each expressed some unique proteins, number multiple lines, but patterns did not always follow traditional clinical definitions type. From our data, can be deduced cells possess strategies promote growth, aberrant expression tyrosine kinases, cellular adhesion molecules, structural proteins. Our data set provides very rich complex picture present on cells, sorting categorizing interesting insights into biology, classification, potential treatment prevalent disease.