Effects of Cobalt Nanoparticles on Human T Cells In Vitro
作者： Haitao Jiang , Fan Liu , Huilin Yang , Yang Li
DOI: 10.1007/S12011-011-9221-8
关键词:
摘要: Limited information is available on the potential risk of degradation products metal-on-metal bearings in joint arthroplasty. The aim this study was to investigate cytotoxicity and genotoxicity orthopedic-related cobalt nanoparticles human T cells vitro. were collected using magnetic CD3 microbeads exposed different concentrations chloride. Cytotoxicity evaluated by methyl thiazolyl tetrazolium lactate dehydrogenase release assay. Cobalt dissolution culture medium determined inductively coupled plasma-mass spectrometry. To probable mechanism effects cells, superoxide dismutase, catalase, glutathione peroxidase level measured. ions could inhibit cell viability enhance a concentration- time-dependent manner (P < 0.05). levels ion released from less than 40% increased with concentration. induce primary DNA damage concentration-dependent manner, caused heavier that ions. exposure significantly decrease activities at subtoxic (6 μM,
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