Regional serotonin metabolism in the brain of transgenic mice lacking monoamine oxidase A.

摘要: The effect of a lack the gene encoding monoamine oxidase A (MAO A) in transgenic Tg8 mice on activity tryptophan hydroxylase (TPH), rate-limiting enzyme serotonin (5-HT) biosynthesis, and levels 5-HT 5-hydroxyindoleacetic acid (5-HIAA) midbrain, hypothalamus, hippocampus, striatum, amygdala, frontal cortex was studied. It shown that with genetic MAO knockout differed from initial C3H/HeJ strain having higher level lower its metabolite, 5-HIAA, all brain regions but cortex, where changes were insignificant. Although 5-HIAA/5-HT ratio various considerably, decrease oxidative deamination index similar different (to 41–45% control values), exception somewhat smaller 54%). presence remaining 45% ± 1.9% value indicates rather effective explains severe behavioral pathological consequences deficiency. An increase TPH lacking found amygdala. No significant midbrain. data show an mutation indicate uniform catabolism except for which is more resistant to than other structures. J. Neurosci. Res. 66:423–427, 2001. © 2001 Wiley-Liss, Inc.