Lack of Uptake and Oxidation of Chylomicron Triglyceride to Carbon Dioxide and Ketone Bodies by the Perfused Rat Liver

摘要: FATTY acids concerned with supplying the caloric requirements of body are transported in blood primarily as free fatty (FFA) or triglyceride (TGFA). The liver is thought to play a central part metabolism both chemical forms1. Investigations have shown that injected FFA labelled carbon-14 taken up by liver2, where they may be catabolized β-oxidation carbon dioxide ketone bodies3, synthesized into TGFA, which give rise very-low-density (d < 1.006) lipoproteins plasma2. Chylomicron-TGFA also reported liver4 and oxidized bodies certain experimental conditions3. However, previous experiments vivo demonstrated magnitude ketogenesis fat-fed rats not related extent influx chylomicrons5 but rather level circulating FFA6. In perfused livers from fasted rats, Morris3 found 75 per cent chylomicron-TGFA were removed perfusate more than 20 4 h. Similarly, Rodbell et al.7 25 uptake 2 oxidation emulsion As these authors used heparin (a known activator lipoprotein lipase) their standard preparations, hydrolysis infused TGFA was responsible for significant rates remains unknown.