Osmoconditioning prevents the onset of microtubular cytoskeleton and activation of cell cycle and is detrimental for germination of Jatropha curcas L. seeds

摘要: Jatropha curcas is an oilseed crop renowned for its tolerance to a diverse range of environmental stresses. In Brazil, this species grown in semiarid regions where establishment requires better understanding the mechanisms underlying appropriate seed, seedling and plant behaviour under water restriction conditions. context, objective study was investigate physiological cytological profiles J. curcas seeds response imbibition (control) polyethylene glycol solution (osmoticum). Seed germinability reactivation cell cycle events were assessed by means different germination parameters immunohistochemical detection tubulin microtubules, i.e. accumulation microtubular cytoskeleton configurations imbibed osmoticum. Immunohistochemical analysis revealed increasing appearance seed embryo radicles from 48 h onwards. Mitotic microtubules only visible water, after radicle protrusion, as indication proliferation, with subsequent root development. Imbibition osmoticum prevented activation cycle, therefore could not be resumed. Osmoconditioned able survive re-drying resume re-imbibition however, lower performance, possibly due acquisition secondary dormancy. This provides important insights into aspects