Expression of cell-cycle-regulating genes in the development of atherosclerosis in Japanese quail (Coturnix japonica).

摘要: The levels of mRNA expression in regulatory genes that are involved the pathological changes aortic atherosclerotic and fibroblastic intimal thickening was investigated Japanese quail. quail were divided into a control diet group an atherogenic group. euthanized at 2, 4, 8, 12 wk after consuming either or diet. Thereafter, both histological immunohistochemical studies analysis cell-cycle-regulating lesions performed on selected ascending aortas their large branches. In group, lipid-containing atheromatous seen mainly 8 wk, respectively. Semiquantitative reverse-transcription PCR used to analyze alterations development lesions. Messenger RNA c-fos c-src showed peak However, no significant alteration c-jun noted during entire experimental period. According progression lesions, c-myc increased chronologically, highest level observed wk. Alterations proliferating cell nuclear antigen p27 gene similar c-myc. c-myc, antigen, significantly correlated with degree lesion our experiment.