Purification of Cu/Zn Superoxide Dismutase fromPiper betleLeaf and Its Characterization in the Oral Cavity

作者: Yu-Ching Liu , Miau-Rong Lee , Chao-Jung Chen , Yung-Chang Lin , Heng-Chien Ho

DOI: 10.1021/JF505753D

关键词:

摘要: The aim of this study was to purify protein(s) from Piper betle leaf for identification and further characterization. A functionally unknown protein purified apparent homogeneity with a molecular mass 15.7 kDa identified as Cu/Zn superoxide dismutase (SOD). SOD appeared be monomeric converted its dimeric form increased enzymatic activity in betel nut oral extract. This irreversible conversion mainly induced by slaked lime, resulting the increase pH cavity. Oral extract chewing areca alone also dimerization due presence arginine. enhanced dimer responsible continuous production hydrogen peroxide Thus, may contribute carcinogenesis through formation cavity, spite protective role against cancer vivo.

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