Glycoprotein emulsifiers from two marine Halomonas species: chemical and physical characterization.

作者: T. Gutiérrez , B. Mulloy , K. Black , D. H. Green

DOI: 10.1111/J.1365-2672.2007.03407.X

关键词:

摘要: Aims:  To partially purify and characterize bioemulsifiers produced by two new marine Halomonas species, TG39 TG67, to compare their emulsifying activities with those of commercial emulsifiers. Methods Results:  The production emulsifiers HE39 HE67 was achieved from glucose-supplemented broth, recovered cell removal, concentration ultrafiltration, precipitation salt ethanol, lyophilization. Purification chemical analysis revealed both be glycoproteins high molecular weight a notably content protein uronic acids. Physical characterization showed effectively emulsify wide range food oils under neutral acidic pH conditions withstand acid temperature treatment. Conclusions:  these glycoprotein were comparable and, certain conditions, superior tested (xanthan gum, gum arabic lecithin). They show the highest reported derived species. Significance Impact Study:  These strains, produced, appear promising candidates for further development in applications requiring that are natural compatible existing emulsifiers.

参考文章(40)
P. D. Patel, J. C. Fry, The search for standardised methods for assessing protein functionality. Developments in Food Proteins. ,vol. 5, pp. 299- 333 ,(1987)
AF Kennedy, IW Sutherland, Analysis of bacterial exopolysaccharides Biotechnology and Applied Biochemistry. ,vol. 9, pp. 12- 19 ,(1987) , 10.1111/J.1470-8744.1987.TB00458.X
M C Cirigliano, G M Carman, Isolation of a bioemulsifier from Candida lipolytica. Applied and Environmental Microbiology. ,vol. 48, pp. 747- 750 ,(1984) , 10.1128/AEM.48.4.747-750.1984
N Kaplan, Z Zosim, E Rosenberg, Reconstitution of emulsifying activity of Acinetobacter calcoaceticus BD4 emulsan by using pure polysaccharide and protein. Applied and Environmental Microbiology. ,vol. 53, pp. 440- 446 ,(1987) , 10.1128/AEM.53.2.440-446.1987
David G. Cooper, Beena G. Goldenberg, Surface-Active Agents from Two Bacillus Species Applied and Environmental Microbiology. ,vol. 53, pp. 224- 229 ,(1987) , 10.1128/AEM.53.2.224-229.1987
Marianne Graber, André Morin, Francis Duchiron, Pierre F. Monsan, Microbial polysaccharides containing 6-deoxysugars Enzyme and Microbial Technology. ,vol. 10, pp. 198- 206 ,(1988) , 10.1016/0141-0229(88)90067-1
I.C.M. Dea, J.K. Madden, ACETYLATED PECTIC POLYSACCHARIDES OF SUGAR BEET Food Hydrocolloids. ,vol. 1, pp. 71- 88 ,(1986) , 10.1016/S0268-005X(86)80009-1
Rachel Shepherd, John Rockey, Ian W. Sutherland, Sibel Roller, Novel bioemulsifiers from microorganisms for use in foods. Journal of Biotechnology. ,vol. 40, pp. 207- 217 ,(1995) , 10.1016/0168-1656(95)00053-S