Detection of cytokeratins in normal and malignant laryngeal epithelia by means of reverse transcriptase-polymerase chain reaction.

摘要: Cytokeratins (CKs) are a subgroup of intermediate filament proteins that take part in forming the cytoskeleton. The epithelial cells different organs express distinct CKs, and this expression may be modified during malignant transformation. Here we describe use reverse transcriptase-polymerase chain reaction followed by Southern blotting to determine profile CKs both normal laryngeal samples. Thirty-six samples were subjected histologic examination classified as 17 squamous cell carcinomas, 3 dysplastic lesions, 16 CK8 CK19 expressed almost all samples, cancerous normal, therefore used verify integrity RNA. Expression CK2, CK9, CK20 was not detected any or cancerous. CK15 CK18 showed low sensitivity for detection cancer (36.4% 45.5%, respectively). CK10 relatively high (91%), but only moderate specificity (69.2%). Only CK17 (91% 92.3%, respectively; positive predictive value, 91%). We propose considered promising candidate molecular marker transformation carcinoma.