Mammary-specific ablation of Cyp24a1 inhibits development, reduces proliferation and increases sensitivity to vitamin D.

摘要: Active vitamin D (1,25(OH)2D) has been shown to regulate numerous cell processes in mammary cells. Degradation of 1,25(OH)2D is initiated by the mitochondrial enzyme, 25-hydroxyvitamin 24-hydroxylase (CYP24 A1), and provides local control bioactivity. Several reports association between elevated CYP24 A1 activity breast cancer incidence, suggest that may be a target for therapeutic intervention. Whether within epithelium regulates levels gland development yet shown. We have used conditional knockout Cyp24a1 gene specifically demonstrate reduced terminal end bud number, ductal outgrowth branching during puberty alveologenesis at early pregnancy, inhibiting proliferation but not apoptosis both basal luminal MECs. In vitro study showed increased sensitivity MECs lower with ablation activity. summary, plays an important role modulating postnatal pregnancy-associated which support as potential approach activating pathway prevention therapy.