Blocking the flow of propionate into TCA cycle through a mutB knockout leads to a significant increase of erythromycin production by an industrial strain of Saccharopolyspora erythraea
作者： Chongchong Chen , Ming Hong , Ju Chu , Mingzhi Huang , Liming Ouyang
DOI: 10.1007/S00449-016-1687-5
关键词:
摘要: A high erythromycin producing mutant strain Saccharopolyspora erythraea HL3168 E3-ΔmutB was constructed by deleting mutB (SACE_5639) gene encoding the beta subunit of methylmalonyl-CoA mutase an industrial S. E3. Industrial media and process control strategies were adopted in a 5 L bioreactor for characterizing physiological parameters. The total titer concentration 46.9 (12740.5 μg/mL) 64.9 % (8094.4 higher than those original strain, respectively, which comparable to production. specific glucose n-propanol consumption rates increased 52.4 39.8 %, respectively. During rapid synthesis phase, yield on also from 24.3 group 66.9 group. Meanwhile, formation propionyl-CoA, two crucial precursors synthesis, 1.89- 2.02-folds
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