ANTIQUORUM SENSING AND ANTIMICROBIAL ACTIVITY OF NATURAL AGENTS WITH POTENTIAL USE IN FOOD

作者: MARíA V. ALVAREZ , MARíA R. MOREIRA , ALEJANDRA PONCE

DOI: 10.1111/J.1745-4565.2012.00390.X

关键词:

摘要: Quorum sensing (QS) is a bacterial communication mechanism that depends on population density. QS inhibitory activity of four bioactive agents and two essential oils was evaluated using the bacteria model Chromobacterium violaceum. On other hand, antimicrobial tested foodborne pathogens, Listeria monocytogenes Escherichia coli. Agents were able to inhibit violacein production, being tea tree rosemary more effective than propolis pollen, resveratrol pomegranate. Tea oils, applied at 0.5 μL/mL, reduced production by 80%. In order evaluate whether inhibition owed microbial growth reduction or acylated homoserine lactone inhibition, analyzed C. violaceum as indicator. None showed minimal concentration. varying degrees antibacterial against pathogens. resulted others. These bacteriostatic effect pathogen counts low concentrations (0.5–1 μL/mL). When they higher 2 μL/mL, bactericidal observed E. coli L. monocytogenes. findings strongly suggest these have potential be good preservatives because anti-QS capacity properties. Practical Applications Food spoilage complex process, excessive amounts foods are lost due even with modern day preservation techniques. Another problem related public health contamination human microorganisms. cell-to-cell which measures its cell Because many important animal plant pathogens use regulate virulence, interruption broad applicability for biological control disease-causing organisms. It considered strategy reduce prevent reaction in foods. this work, we investigated (using violaceum) some extracts oils. The results obtained from study indicate could potentially used natural prolong shelf life ensure safety

参考文章(32)
Toshiko Hada, Yoshihiro Inoue, Akiko Shiraishi, Hajime Hamashima, Leakage of K+ ions from Staphylococcus aureus in response to tea tree oil. Journal of Microbiological Methods. ,vol. 53, pp. 309- 312 ,(2003) , 10.1016/S0167-7012(02)00248-8
V.A. Pandit, L.A. Shelef, Sensitivity of Listeria monocytogenes to rosemary (Rosmarinus officinalis L.) Food Microbiology. ,vol. 11, pp. 57- 63 ,(1994) , 10.1006/FMIC.1994.1008
J. Fernández-López, N. Zhi, L. Aleson-Carbonell, J.A. Pérez-Alvarez, V. Kuri, Antioxidant and antibacterial activities of natural extracts: application in beef meatballs Meat Science. ,vol. 69, pp. 371- 380 ,(2005) , 10.1016/J.MEATSCI.2004.08.004
Khee Hoon Koh, Foong-Yee Tham, Screening of traditional Chinese medicinal plants for quorum-sensing inhibitors activity Journal of Microbiology Immunology and Infection. ,vol. 44, pp. 144- 148 ,(2011) , 10.1016/J.JMII.2009.10.001
D.A. Vattem, K. Mihalik, S.H. Crixell, R.J.C. McLean, Dietary phytochemicals as quorum sensing inhibitors. Fitoterapia. ,vol. 78, pp. 302- 310 ,(2007) , 10.1016/J.FITOTE.2007.03.009
P. Truchado, F. López-Gálvez, M.I. Gil, F.A. Tomás-Barberán, A. Allende, Quorum sensing inhibitory and antimicrobial activities of honeys and the relationship with individual phenolics Food Chemistry. ,vol. 115, pp. 1337- 1344 ,(2009) , 10.1016/J.FOODCHEM.2009.01.065
Brahma N. Singh, B.R. Singh, R.L. Singh, D. Prakash, B.K. Sarma, H.B. Singh, Antioxidant and anti-quorum sensing activities of green pod of Acacia nilotica L. Food and Chemical Toxicology. ,vol. 47, pp. 778- 786 ,(2009) , 10.1016/J.FCT.2009.01.009
Gulgun Bosgelmez-Tinaz, Seyhan Ulusoy, Aysel Ugur, Ozgur Ceylan, Inhibition of quorum sensing-regulated behaviors by Scorzonera sandrasica. Current Microbiology. ,vol. 55, pp. 114- 118 ,(2007) , 10.1007/S00284-006-0624-2
S.F. Bodini, S. Manfredini, M. Epp, S. Valentini, F. Santori, Quorum sensing inhibition activity of garlic extract and p-coumaric acid. Letters in Applied Microbiology. ,vol. 49, pp. 551- 555 ,(2009) , 10.1111/J.1472-765X.2009.02704.X