Generation and Characterization of Murine Monoclonal Antibodies anti-PBP2a of Methicillin-resistant Staphylococcus aureus
作者： José Procópio M. Senna , Maria da Glória M. Teixeira , Marta de A. Santiago , Nádia M. Batoréu , Napoleão Valadares
关键词:
摘要: Methicillin-resistant Staphylococcus aureus (MRSA) is a multidrug-resistant bacterium that causes serious infections worldwide. This pathogen resistant to all beta lactam antibiotics due the presence of PBP2a, transpeptidase enzyme presents very low beta-lactam affinity. Here we report generation and characterization mouse monoclonal antibodies PBP2a MRSA strains. Two clones were obtained characterized by immunoassays (ELISA, avidity index determination, immunoblotting), isotyping, association/dissociation rate constants surface plasmon resonance (SPR), flow cytometry. Clone 38, which showed best affinity, bound located on bacterial Further studies are warranted in order evaluate if these may help inhibit growth be used treat MRSA.
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