The Binding of Detergents to Lipophilic and Hydrophilic Proteins
作者： Ari Helenius , Kai Simons
DOI: 10.1016/S0021-9258(19)45190-9
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摘要: Abstract The lipophilic protein moieties of human low density lipoprotein, Semliki Forest virus envelope, and erythrocyte stroma were obtained lipid-free with the use sodium deoxycholate treatment gel filtration in deoxycholate-containing media. Detergent binding was determined by subjecting proteins to presence micellar concentrations radioactive or nonionic detergent Triton X-100. amount detergents bound could be measured from that co-eluted protein. large amounts X-100 (up about 70% their weight). hydrophilic tested little no
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