Recombinant IκB Kinases α and β Are Direct Kinases of IκBα
作者： Jun Li , Gregory W. Peet , Steven S. Pullen , Josephine Schembri-King , Thomas C. Warren
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摘要: Activation of the transcription factor NF-κB is regulated by phosphorylation and subsequent degradation its inhibitory subunit, IκB. A large multiprotein complex, IκB kinase (IKK), catalyzes The two components IKK IKKα IKKβ, were overexpressed in insect cells purified to homogeneity. Both IKKβ specifically catalyzed regulatory serine residues IκBα. Hence, functional catalytic subunits complex. Purified also preferentially phosphorylated as opposed threonine IκBα, consistent with substrate preference Kinetic analysis revealed that activity on IκBα 50–60-fold higher than IKKα. primary difference between activities K m for kinetics both followed a sequential Bi mechanism. No synergistic effects detected IKKβ. Thus, vitro, are independent kinases
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