DNA double-strand break induction and rejoining as determinants of human tumour cell radiosensitivity. A pulsed-field gel electrophoresis study.

摘要: Measurement of the surviving fraction after 2 Gy (SF2) may predict for local control tumour and patients cure, but clonogenic assays are unsuitable wider clinical application. Promising results have been obtained using DNA damage such as pulsed-field gel electrophoresis, PFGE. In current study, nine human cell lines (SF2, range 0·08–0·62) were studied double-strand break (dsb) induction six these dsb rejoining Differences in induction, slope (±SEM) release per Gy, varied from 1·30 (0·05) to 2·42 (0·17). The frequency 3·55 (0·33) 9·69 (2·18) × 10−9/bp/Gy (21–56 dsb/Gy/cell). Variations half-time fast phase (18–60 min) slow (38–445 observed. Statistically significant correlations found between SF2 curve (p = 0·003), 10 0·029) 20 0·011) 0·012). Wh...